IL12 antibody (Interleukin 12)

Details for Product anti-IL12 Antibody No. ABIN6730996
Antigen
Reactivity
Human
112
49
17
15
9
5
2
2
2
2
2
2
1
1
1
Host
Mouse
70
63
40
19
2
1
Antibody Type
Recombinant Antibody
Clonality (Clone)
Monoclonal ()
Conjugate
This IL12 antibody is un-conjugated
35
11
9
6
6
4
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
127
126
34
31
26
24
20
16
16
13
7
7
6
5
3
2
2
2
2
2
2
1
1
1
1
1
Options
Purpose Ustekinumab is a fully human IgG1κ monoclonal antibody that binds with specificity to the shared p40 protein subunit of human cytokines interleukin (IL)-12 and IL-23.
Clone Ustekinumab
Specificity "Ustekinumab is a fully human IgG1κ monoclonal antibody that binds with specificity to the shared p40 protein subunit of human cytokines interleukin (IL)-12 and IL-23."
Characteristics "This is a therapeutic antibody derived from Stelara and designed for research purposes. Ustekinumab, the active ingredient of Stelara, is a fully human IgG1κ monoclonal antibody that binds with specificity to the shared p40 protein subunit of human cytokines interleukin (IL)-12 and IL-23."
Ustekinumab is a fully human IgG1κ monoclonal antibody that binds with specificity to the shared p40 protein subunit of human cytokines interleukin (IL)-12 and IL-23. Ustekinumab inhibits the bioactivity of human IL-12 and IL-23 by preventing p40 from binding to the IL-12R1 receptor protein expressed on the surface of immune cells. Ustekinumab cannot bind to IL-12 or IL-23 that is already bound to IL-12R1 cell surface receptors. Thus, ustekinumab is not likely to contribute to complement- or antibody-mediated cytotoxicity of cells with IL-12 and/or IL-23 receptors. IL-12 and IL-23 are heterodimeric cytokines secreted by activated antigen presenting cells, such as macrophages and dendritic cells, and both cytokines participate in immune functions; IL-12 stimulates natural killer (NK) cells and drives the differentiation of CD4+ T cells toward the T helper 1 (Th1) phenotype, IL- 23 induces the T helper 17 (Th17) pathway. However, abnormal regulation of IL 12 and IL 23 has been associated with immune mediated diseases, such as psoriasis, psoriatic arthritis and Crohn’s disease.
By binding the shared p40 subunit of IL-12 and IL-23, ustekinumab may exert its clinical effects in psoriasis, psoriatic arthritis and Crohn’s disease through interruption of the Th1 and Th17 cytokine pathways, which are central to the pathology of these diseases.
In patients with Crohn’s disease, treatment with ustekinumab resulted in a decrease in inflammatory markers including C-Reactive Protein (CRP) and fecal calprotectin during the induction phase, which were then maintained throughout the maintenance phase.
Purification The commercial therapeutic mAb was diluted with sterile PBS to a final 1 mg/ml.  (original drug Stelara 90 was diluted 1:90)
Sterility Sterile
Antigen
Alternative Name IL-12 (IL12 Antibody Abstract)
Background Synonyms: CLMF, CLMF2, IL-12B, NKSF, NKSF2, p40, Il-12b, Il12p40, Il-12p40, Il12, IL-12p40, LOC100217488, IL-12p35, Il-12a, Ll12a, p35, IL12p40, IL12B, IL-12 p35
Molecular Weight 37 kDa
Gene ID 3592
UniProt P29459
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints
Application Notes Optimal dilution for a specific application should be determined by user
Comment

Ustekinumab is a fully human IgG1κ monoclonal antibody to interleukin(IL)-12/23 produced in a murine myeloma cell line using recombinant DNA technology

Protocol Research-relevant quantities are available as licence-free consumable as diluted variant. Therapeutic monoclonal antibodies can be used from researchers in in vitro and in vivo experiments.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer The dilutant is sterile PBS pH 7.4. No preservatives added
Preservative Without preservative
Handling Advice open only under sterile conditions
Storage 4 °C,-20 °C
Storage Comment Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Expiry Date 36 months