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TNF alpha antibody (Tumor Necrosis Factor alpha) Primary Antibody

TNF alpha Reactivity: Human BR, ELISA, FACS, IP, WB Host: CHO Cells Monoclonal Adalimumab (D2E7) unconjugated
Catalog No. ABIN6730997
$360.00
Plus shipping costs $45.00
100 μL
local_shipping Shipping to: United States
Delivery in 7 to 8 Business Days
  • Target
    TNF alpha
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 417
    • 184
    • 139
    • 49
    • 41
    • 35
    • 29
    • 29
    • 23
    • 13
    • 12
    • 12
    • 8
    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 292
    • 263
    • 43
    • 32
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    CHO Cells
    Clonality
    • 341
    • 293
    • 4
    Monoclonal
    Conjugate
    • 328
    • 91
    • 52
    • 22
    • 20
    • 16
    • 8
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    This TNF alpha antibody is un-conjugated
    Application
    • 315
    • 292
    • 199
    • 172
    • 89
    • 65
    • 62
    • 59
    • 53
    • 50
    • 49
    • 45
    • 35
    • 28
    • 19
    • 16
    • 14
    • 10
    • 10
    • 8
    • 7
    • 7
    • 7
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Blocking Reagent (BR), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
    Purpose
    Adalimumab binds specifically to TNF and neutralises the biological function of TNF by blocking its interaction with the p55 and p75 cell surface TNF receptors.
    Specificity
    "Adalimumab binds specifically to TNF and neutralises the biological function of TNF by blocking its interaction with the p55 and p75 cell surface TNF receptors."
    Characteristics
    "This is a therapeutic antibody derived from Humira and designed for research purposes. Adalimumab, the active ingredient of Humira, binds specifically to TNF and neutralises the biological function of TNF by blocking its interaction with the p55 and p75 cell surface TNF receptors."
    Adalimumab binds specifically to TNF and neutralizes the biological function of TNF by blocking its interaction with the p55 and p75 cell surface TNF receptors.
    Adalimumab also modulates biological responses that are induced or regulated by TNF, including changes in the levels of adhesion molecules responsible for leukocyte migration (ELAM-1, VCAM-1, and ICAM-1 with an IC50 of0.1-0.2 nM).
    Purification
    The commercial therapeutic mAb was diluted with sterile PBS to a final 1 mg/ml.  (original drug Humira 40 was diluted 1:100)
    Sterility
    Sterile
    Clone
    Adalimumab (D2E7)
  • Application Notes
    Optimal dilution for a specific application should be determined by user
    Comment

    Adalimumab is a recombinant human monoclonal antibody expressed in Chinese Hamster Ovary cells.

    Protocol
    Research-relevant quantities are available as licence-free consumable as diluted variant. Therapeutic monoclonal antibodies can be used from researchers in in vitro and in vivo experiments.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    The dilutant is sterile PBS pH 7.4. No preservatives added
    Preservative
    Without preservative
    Handling Advice
    open only under sterile conditions
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
    Expiry Date
    24 months
  • Target
    TNF alpha
    Alternative Name
    TNF-alpha (TNF alpha Antibody Abstract)
    Synonyms
    DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa
    Background
    Synonyms: DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin
    Molecular Weight
    26 kDa
    Gene ID
    21926
    NCBI Accession
    NM_000594
    UniProt
    P01375
    Pathways
    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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