GABRB2 antibody (AA 143-192)

Catalog No. ABIN6736385

This Rabbit Polyclonal antibody specifically detects GABRB2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Pig, Chicken, Monkey, Bat and Xenopus laevis.

Quick Overview for GABRB2 antibody (AA 143-192) (ABIN6736385)

Target: GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))

Reactivity: Human, Rat, Mouse, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Pig, Chicken, Monkey, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GABRB2 Antibody

Binding Specificity: AA 143-192

Specificity: Human GABRB2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human GABRB2 (P47870, NP_000804). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Cat, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Frog, Stickleback (100%), Dog (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GABRB2

Target: GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))

Alternative Name: GABRB2

Background: Name/Gene ID: GABRB2
Subfamily: GABA A receptor
Family: Ion Channel

Synonyms: GABRB2, GABA A receptor beta 2, GABA(A) receptor, beta 2

Gene ID: 2561

NCBI Accession: NP_000804

UniProt: P47870

Pathways: Sensory Perception of Sound, Synaptic Membrane