CDX2 antibody (AA 161-210)

Catalog No. ABIN214089

Product Details anti-CDX2 Antibody

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Binding Specificity: AA 161-210

Reactivity: Human, Mouse, Rat, Monkey, Dog, Pig, Rabbit, Guinea Pig, Bat, Chicken, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CDX2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa161-210 of human CDX2 (Q99626, NP_001256). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Rabbit, Pig, Guinea pig, Chicken (100%), Opossum, Zebra finch (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CDX2

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Alternative Name: CDX2 (CDX2 Products)

Synonyms: CDX2 antibody, MGC130987 antibody, cdx3-like antibody, CDX-3 antibody, CDX3 antibody, CDX-2 antibody, Cdx-2 antibody, caudal type homeobox 2 antibody, caudal type homeobox 2 L homeolog antibody, caudal type homeo box 2 antibody, CDX2 antibody, cdx2.L antibody, Cdx2 antibody

Background: Name/Gene ID: CDX2

Synonyms: CDX2, Caudal-type homeobox protein 2, CDX3, Homeobox protein CDX-2, Caudal type homeobox 2, CDX-3

Gene ID: 1045

NCBI Accession: NP_001256

UniProt: Q99626

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance