AFG3L2 antibody (AA 324-373)

Catalog No. ABIN6740990

Product Details anti-AFG3L2 Antibody

Target: AFG3L2 (AFG3-Like Protein 2 (AFG3L2))

Binding Specificity: AA 324-373

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Guinea Pig, Horse, Pig, Xenopus laevis, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFG3L2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human AFG3L2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea pig (100%) Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa324-373 of human AFG3L2 (Q9Y4W6, NP_006787). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%), Turkey, Chicken, Stickleback (92%), Drosophila, Arabidopsis (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AFG3L2

Target: AFG3L2 (AFG3-Like Protein 2 (AFG3L2))

Alternative Name: AFG3L2 (AFG3L2 Products)

Synonyms: MGC147390 antibody, si:ch211-12e1.4 antibody, SCA28 antibody, SPAX5 antibody, 2310036I02Rik antibody, AW260507 antibody, Emv66 antibody, par antibody, AFG3 like matrix AAA peptidase subunit 2 antibody, AFG3-like protein 2 antibody, AFG3 ATPase family gene 3-like 2 (S. cerevisiae) antibody, AFG3-like AAA ATPase 2 antibody, AFG3-like AAA ATPase 2 L homeolog antibody, AFG3L2 antibody, LOC578526 antibody, afg3l2 antibody, afg3l2.L antibody, Afg3l2 antibody

Background: Name/Gene ID: AFG3L2
Subfamily: Metallopeptidase M41
Family: Protease

Synonyms: AFG3L2, AFG3-like protein 2, SPAX5, Spinocerebellar ataxia 28, Paraplegin-like protein, ATPase family gene 3, yeast, SCA28

Gene ID: 10939

NCBI Accession: NP_006787

UniProt: Q9Y4W6

Pathways: Skeletal Muscle Fiber Development