Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

TCF7L1 antibody (AA 35-84)

TCF7L1 Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741527
  • Target See all TCF7L1 Antibodies
    TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))
    Binding Specificity
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 35-84
    Reactivity
    • 34
    • 16
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Rabbit, Guinea Pig
    Host
    • 32
    • 1
    • 1
    Rabbit
    Clonality
    • 33
    • 1
    Polyclonal
    Conjugate
    • 18
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This TCF7L1 antibody is un-conjugated
    Application
    • 18
    • 9
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human TCF7L1
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Guinea pig (100%) Rabbit (85%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa35-84 of human TCF7L1 (Q9HCS4, NP_112573). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Bovine, Guinea pig (100%), Monkey, Elephant, Opossum (92%), Rabbit (85%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product TCF7L1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))
    Alternative Name
    TCF7L1 (TCF7L1 Products)
    Synonyms
    TCF-3 antibody, TCF3 antibody, Tcf-3 antibody, Tcf3 antibody, bHLHb21 antibody, tcf3c antibody, tcf3d antibody, xtcf3 antibody, XTCF-3 antibody, tcf7l1 antibody, tcf3b antibody, TCF-3-A antibody, tcf-3 antibody, tcf3 antibody, tcf3a antibody, zTcf-3 antibody, transcription factor 7 like 1 antibody, transcription factor 7 like 1 (T cell specific, HMG box) antibody, transcription factor 7 like 1 L homeolog antibody, transcription factor 7 like 1 S homeolog antibody, transcription factor 7 like 1b antibody, transcription factor 7 like 1a antibody, TCF7L1 antibody, Tcf7l1 antibody, tcf7l1.L antibody, tcf7l1.S antibody, tcf7l1b antibody, tcf7l1a antibody
    Background
    Name/Gene ID: TCF7L1

    Synonyms: TCF7L1, TCF-3, Transcription factor 7-like 1, HMG box transcription factor 3
    Gene ID
    83439
    NCBI Accession
    NP_112573
    UniProt
    Q9HCS4
    Pathways
    WNT Signaling, Stem Cell Maintenance
Support