TCF7L1 antibody (AA 35-84)
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- Target See all TCF7L1 Antibodies
- TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))
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Binding Specificity
- AA 35-84
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Reactivity
- Human, Mouse, Rat, Cow, Rabbit, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This TCF7L1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human TCF7L1
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Guinea pig (100%) Rabbit (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa35-84 of human TCF7L1 (Q9HCS4, NP_112573). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Bovine, Guinea pig (100%), Monkey, Elephant, Opossum (92%), Rabbit (85%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product TCF7L1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))
- Alternative Name
- TCF7L1 (TCF7L1 Products)
- Synonyms
- TCF-3 antibody, TCF3 antibody, Tcf-3 antibody, Tcf3 antibody, bHLHb21 antibody, tcf3c antibody, tcf3d antibody, xtcf3 antibody, XTCF-3 antibody, tcf7l1 antibody, tcf3b antibody, TCF-3-A antibody, tcf-3 antibody, tcf3 antibody, tcf3a antibody, zTcf-3 antibody, transcription factor 7 like 1 antibody, transcription factor 7 like 1 (T cell specific, HMG box) antibody, transcription factor 7 like 1 L homeolog antibody, transcription factor 7 like 1 S homeolog antibody, transcription factor 7 like 1b antibody, transcription factor 7 like 1a antibody, TCF7L1 antibody, Tcf7l1 antibody, tcf7l1.L antibody, tcf7l1.S antibody, tcf7l1b antibody, tcf7l1a antibody
- Background
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Name/Gene ID: TCF7L1
Synonyms: TCF7L1, TCF-3, Transcription factor 7-like 1, HMG box transcription factor 3 - Gene ID
- 83439
- NCBI Accession
- NP_112573
- UniProt
- Q9HCS4
- Pathways
- WNT Signaling, Stem Cell Maintenance
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