NR2E1 antibody (AA 95-144)

Catalog No. ABIN6741553

Product Details anti-NR2E1 Antibody

Target: NR2E1 (Nuclear Receptor Subfamily 2, Group E, Member 1 (NR2E1))

Binding Specificity: AA 95-144

Reactivity: Human, Dog, Horse, Cow, Rabbit, Pig, Monkey, Bat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR2E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NR2E1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Mouse, Rat, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa95-144 of human NR2E1 (Q9Y466, NP_003260). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Shrew, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Sparrow, Chicken, Armadillo (100%), Mouse, Rat, Guinea pig, Zebra finch, Platypus, Lizard, Xenopus, Stickleback, Medaka, Pufferfish, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NR2E1

Target: NR2E1 (Nuclear Receptor Subfamily 2, Group E, Member 1 (NR2E1))

Alternative Name: NR2E1 / TLX (NR2E1 Products)

Background: Name/Gene ID: NR2E1
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR

Synonyms: NR2E1, HTll, Tailless homolog, Tailless, TLX, TLL, Nuclear receptor TLX, Protein tailless homolog, Tailes-related receptor, XTLL

Gene ID: 7101

NCBI Accession: NP_003260

UniProt: Q9Y466

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance