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SCML1 antibody (AA 108-157)

SCML1 Reactivity: Human, Pig, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741563
  • Target See all SCML1 Antibodies
    SCML1 (Sex Comb On Midleg-Like 1 (SCML1))
    Binding Specificity
    • 8
    • 8
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 108-157
    Reactivity
    • 28
    • 1
    • 1
    • 1
    Human, Pig, Monkey
    Host
    • 23
    • 5
    Rabbit
    Clonality
    • 25
    • 3
    Polyclonal
    Conjugate
    • 18
    • 2
    • 2
    • 2
    • 2
    • 2
    This SCML1 antibody is un-conjugated
    Application
    • 28
    • 17
    • 6
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human SCML1
    Predicted Reactivity
    Percent identity by BLAST analysis: Human (100%) Pig (85%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa108-157 of human SCML1 (Q5H968, NP_006737). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Pig (100%), Chimpanzee, Gibbon (92%), Elephant (84%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product SCML1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    SCML1 (Sex Comb On Midleg-Like 1 (SCML1))
    Alternative Name
    SCML1 (SCML1 Products)
    Synonyms
    Scm polycomb group protein like 1 antibody, SCML1 antibody
    Background
    Name/Gene ID: SCML1

    Synonyms: SCML1
    Gene ID
    6322
    NCBI Accession
    NP_006737
    UniProt
    Q9UN30
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