NR0B2 antibody (AA 110-159)

Catalog No. ABIN6741607

Product Details anti-NR0B2 Antibody

Target: NR0B2 (Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2))

Binding Specificity: AA 110-159

Reactivity: Human, Rabbit, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR0B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NR0B2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog (100%) Horse (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa110-159 of human NR0B2 (Q15466, NP_068804). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Tamarin, Dog, Rabbit (100%), Baboon, Galago (92%), Horse (91%), Elephant, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NR0B2

Target: NR0B2 (Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2))

Alternative Name: NR0B2 / SHP (NR0B2 Products)

Synonyms: SHP antibody, SHP1 antibody, SHP-1 antibody, Shp1 antibody, Shp antibody, NR0B2-A antibody, Nr0b2 antibody, SHP-A antibody, gb:bc058069 antibody, nuclear receptor subfamily 0 group B member 2 antibody, nuclear receptor subfamily 0, group B, member 2 antibody, nuclear receptor subfamily 0, group B, member 2a antibody, NR0B2 antibody, Nr0b2 antibody, nr0b2a antibody

Background: Name/Gene ID: NR0B2
Subfamily: NR0 Knirps-like
Family: NHR

Synonyms: NR0B2, Short heterodimer partner, SHP, Nuclear receptor SHP, Orphan nuclear receptor SHP, Small heterodimer partner, SHP1

Gene ID: 8431

NCBI Accession: NP_068804

UniProt: Q15466

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway