NR1H4 antibody (AA 323-372)

Catalog No. ABIN6741866

Product Details anti-NR1H4 Antibody

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Binding Specificity: AA 323-372

Reactivity: Human, Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR1H4 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human NR1H4

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken (92%) Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa323-372 of human NR1H4 (B6ZGS9, NP_005114). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (92%), Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NR1H4

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Alternative Name: NR1H4 / FXR (NR1H4 Products)

Synonyms: zgc:110190 antibody, zgc:92742 antibody, NR1H4 antibody, BAR antibody, FXR antibody, HRR-1 antibody, HRR1 antibody, RIP14 antibody, AI957360 antibody, Fxr antibody, Rxrip14 antibody, nuclear receptor subfamily 1, group H, member 5 S homeolog antibody, nuclear receptor subfamily 1, group H, member 4 antibody, nuclear receptor subfamily 1 group H member 4 antibody, nr1h5.S antibody, nr1h4 antibody, NR1H4 antibody, Nr1h4 antibody

Background: Name/Gene ID: NR1H4
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: NR1H4, BAR, Farnesyl x receptor, FXR, HRR1, Farnesoid x activated receptor, Farnesoid X receptor, Farnesol receptor HRR-1, HRR-1, RXR-interacting protein 14, RIP14, Bile acid receptor, Farnesoid X nuclear receptor, Farnesoid X-activated receptor

Gene ID: 9971

NCBI Accession: NP_005114

UniProt: Q96RI1

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Carbohydrate Metabolic Process