ATP6V1B2 antibody (C-Term)

Catalog No. ABIN6744046

The Rabbit Polyclonal anti-ATP6V1B2 antibody has been validated for WB. It is suitable to detect ATP6V1B2 in samples from Human, Mouse, Rat, Cow, Dog, Rabbit, Pig, Horse, Bat and Monkey.

Quick Overview for ATP6V1B2 antibody (C-Term) (ABIN6744046)

Target: ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Pig, Horse, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1B2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6V1B2 Antibody

Binding Specificity: C-Term

Specificity: Human ATP6V1B2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit, Pig (100%) Zebrafish (91%) Xenopus (86%) Chicken (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ATP6V1B2 (P21281, NP_001684). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Platypus (100%), Guinea pig (92%), Zebrafish (91%), Opossum, Beetle (90%), Xenopus (86%), Zebra finch (85%), Trout, Salmon, Stickleback (84%), Turkey, Chicken (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ATP6V1B2

Target: ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))

Alternative Name: ATP6V1B2

Background: Name/Gene ID: ATP6V1B2
Subfamily: ATPase - V type
Family: Transporter

Synonyms: ATP6V1B2, ATP6B1B2, ATP6B2, V-ATPase subunit B 2, Vma2, VATB, VPP3, HO57, V-ATPase B2 subunit

Gene ID: 526

NCBI Accession: NP_001684

UniProt: P21281

Pathways: Transition Metal Ion Homeostasis, Proton Transport