B4GALT5 antibody (AA 311-360)

Catalog No. ABIN6746272

Product Details anti-B4GALT5 Antibody

Target: B4GALT5 (UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 5 (B4GALT5))

Binding Specificity: AA 311-360

Reactivity: Human, Mouse, Rat, Monkey, Cow, Dog, Horse, Pig, Rabbit, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B4GALT5 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse B4GALT5

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig (100%) Guinea pig, Chicken, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa311-360 of mouse B4galt5 (Q9JMK0, NP_062809). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Platypus (100%), Guinea pig, Zebra finch, Chicken, Lizard, Xenopus (92%), Marmoset, Panda, Turkey, Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-B4GALT5 antibody (ABIN6746272)

Dzwonek, Preobrazhenska, Cazzola, Conidi, Schellens, van Dinther, Stubbs, Klippel, Huylebroeck, ten Dijke, Verschueren: "Smad3 is a key nonredundant mediator of transforming growth factor beta signaling in Nme mouse mammary epithelial cells." in: Molecular cancer research : MCR, Vol. 7, Issue 8, pp. 1342-53, (2009) (PubMed).

Hayes, Chawla, Corvera: "TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2." in: The Journal of cell biology, Vol. 158, Issue 7, pp. 1239-49, (2002) (PubMed).

Lehmann, Janda, Pierreux, Rytömaa, Schulze, McMahon, Hill, Beug, Downward: "Raf induces TGFbeta production while blocking its apoptotic but not invasive responses: a mechanism leading to increased malignancy in epithelial cells." in: Genes & development, Vol. 14, Issue 20, pp. 2610-22, (2000) (PubMed).

Target Details for B4GALT5

Target: B4GALT5 (UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 5 (B4GALT5))

Alternative Name: B4GALT5 (B4GALT5 Products)

Synonyms: B4Gal-T5 antibody, BETA4-GALT-IV antibody, beta4Gal-T5 antibody, beta4GalT-V antibody, gt-V antibody, 9430078I07Rik antibody, AW049941 antibody, AW539721 antibody, beta-1,4-galactosyltransferase 5 antibody, UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 antibody, B4GALT5 antibody, B4galt5 antibody

Background: Name/Gene ID: B4GALT5

Synonyms: B4GALT5, Beta-1,4-GalT IV, Beta4-GalT IV, BETA4-GALT-IV, Beta4Gal-T5, Beta-1,4-GalT II, Beta-1,4-GalTase 5, Beta4GalT-V, Gt-V, B4Gal-T5

Gene ID: 9334

NCBI Accession: NP_062809

UniProt: O43286

Pathways: Glycosaminoglycan Metabolic Process