CD51/CD61 antibody

Catalog No. ABIN674784

Product Details anti-CD51/CD61 Antibody

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD51/CD61 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Integrin Alpha V + Beta 3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD51/CD61 antibody (ABIN674784)

Bian, Ma, Jain, Crane, Kebaish, Wan, Zhang, Edward Guo, Sponseller, Séguin, Riley, Wang, Cao: "Mechanosignaling activation of TGFβ maintains intervertebral disc homeostasis." in: Bone research, Vol. 5, pp. 17008, (2017) (PubMed).

Li, Xiong, Gong: "Lyn kinase enhanced hepatic fibrosis by modulating the activation of hepatic stellate cells." in: American journal of translational research, Vol. 9, Issue 6, pp. 2865-2877, (2017) (PubMed).

Chen, Chen, Kemper, Brigstock: "Pathways of production and delivery of hepatocyte exosomes." in: Journal of cell communication and signaling, (2017) (PubMed).

Li, Zhou, Li, Zhang, Shao, Tang, Tian: "Effects of Cationic Microbubble Carrying CD/TK Double Suicide Gene and αVβ3 Integrin Antibody in Human Hepatocellular Carcinoma HepG2 Cells." in: PLoS ONE, Vol. 11, Issue 7, pp. e0158592, (2017) (PubMed).

Chen, Zheng, Liu, Shi, Qi: "Periostin activates pathways involved in epithelial-mesenchymal transition in adamantinomatous craniopharyngioma." in: Journal of the neurological sciences, Vol. 360, pp. 49-54, (2016) (PubMed).

Chen, Brigstock: "Integrins and heparan sulfate proteoglycans on hepatic stellate cells (HSC) are novel receptors for HSC-derived exosomes." in: FEBS letters, Vol. 590, Issue 23, pp. 4263-4274, (2016) (PubMed).

Muschter, Göttl, Vogel, Grifka, Straub, Grässel: "Reactivity of rat bone marrow-derived macrophages to neurotransmitter stimulation in the context of collagen II-induced arthritis." in: Arthritis research & therapy, Vol. 17, pp. 169, (2016) (PubMed).

Jiang, Tu, Kimura, Habte, Chen, Cheng, Shi, Gambhir, Cheng: "64Cu-Labeled Divalent Cystine Knot Peptide for Imaging Carotid Atherosclerotic Plaques." in: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Vol. 56, Issue 6, pp. 939-44, (2015) (PubMed).

Mondal, Barui, Chaudhuri: "The relationship between the cyclic-RGDfK ligand and ?v?3 integrin receptor." in: Biomaterials, Vol. 34, Issue 26, pp. 6249-60, (2013) (PubMed).

Target Details for CD51/CD61

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin AlphaV, Integrin Beta3 (ITGAV/ITGB3))

Alternative Name: Integrin Alpha V + Beta 3 (ITGAV/ITGB3 Products)

Synonyms: CD51 antibody, MSK8 antibody, VNRA antibody, VTNR antibody, integrin subunit alpha V antibody, ITGAV antibody

Background:

Synonyms: CD51, MSK8, VNRA, VTNR, Integrin alpha-V, Vitronectin receptor subunit alpha, ITGAV, CD61, GP3A, BDPLT2, GPIIIa, BDPLT16, Integrin beta-3, Platelet membrane glycoprotein IIIa, ITGB3

Background: ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8, 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands, the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Gene ID: 3690, 3685

UniProt: P05106, P06756