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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) Primary Antibody

GAPDH Reactivity: Human, Mouse, Rat IF (p), IHC (p), WB Host: Rabbit Polyclonal unconjugated
Pubmed (16)
Catalog No. ABIN678458
$339.90
Plus shipping costs $45.00
100 μL
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  • Target
    GAPDH
    Reactivity
    • 296
    • 185
    • 170
    • 78
    • 67
    • 63
    • 35
    • 35
    • 31
    • 27
    • 23
    • 20
    • 20
    • 19
    • 19
    • 15
    • 13
    • 10
    • 9
    • 8
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 192
    • 116
    • 24
    • 12
    • 6
    • 1
    Rabbit
    Clonality
    • 226
    • 124
    Polyclonal
    Conjugate
    • 198
    • 36
    • 24
    • 18
    • 8
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This GAPDH antibody is un-conjugated
    Application
    • 271
    • 134
    • 87
    • 73
    • 51
    • 47
    • 47
    • 38
    • 28
    • 28
    • 21
    • 15
    • 7
    • 6
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purification
    Purified by Protein A.
    Immunogen
    GAPDH protein of rabbit
    Isotype
    IgG
  • Application Notes
    WB 1:100-1000
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C
    Expiry Date
    12 months
  • Zhang, Wang, Xie, Chen, Sinkemani, Yu, Wang, Mao, Wu: "Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence." in: Oncotarget, Vol. 9, Issue 2, pp. 2175-2192, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

    Sun, Zhang, Tang, Yang, Chu, Zhao: "SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer." in: International journal of nanomedicine, Vol. 12, pp. 2657-2672, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

    Tan, Shi, Ba, Lin, Tang, Zeng, Zhang: "miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy." in: International journal of molecular medicine, Vol. 37, Issue 4, pp. 1030-8, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

    Jia, Song, Yang, Ma, Li, Lu, Cao, Zhang, Zhu, Wang, Leng, Cao, Du, Xu: "Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats." in: Molecular medicine reports, Vol. 13, Issue 6, pp. 4627-35, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: Liver

    Wang, Jia, Chen, Wang, Li: "Osthole shows the potential to overcome P-glycoprotein‑mediated multidrug resistance in human myelogenous leukemia K562/ADM cells by inhibiting the PI3K/Akt signaling pathway." in: Oncology reports, Vol. 35, Issue 6, pp. 3659-68, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

    Xiong, Luo, He, Xi, Lu, Li, Liu, Guo: "Up-regulation of miR-138 inhibits hypoxia-induced cardiomyocyte apoptosis via down-regulating lipocalin-2 expression." in: Experimental biology and medicine (Maywood, N.J.), Vol. 241, Issue 1, pp. 25-30, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

    Yang, Yao, Qian, Wei, Wu, Wang: "Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium." in: Cellular and molecular life sciences : CMLS, Vol. 72, Issue 24, pp. 4849-66, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

    Hou, Liu, Chai, Yu, Duan, Hu, Yin, Wang, Zhuang, Fan, Chu, Ma: "Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein." in: Molecular medicine reports, Vol. 12, Issue 2, pp. 2521-8, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

    Zhang, Shi, Lin, Ba, Cui: "MicroRNA-216a enhances the radiosensitivity of pancreatic cancer cells by inhibiting beclin-1-mediated autophagy." in: Oncology reports, Vol. 34, Issue 3, pp. 1557-64, 2015 (PubMed). Method employed by authors: Western Blotting (WB)

    Shao, Qian, Wang, Yan, Hu, Wang, Wang: "Construction of the plasmid coding for the expression of the EGFP-M-IL-2((88)Arg, (125)Ala) fusion protein and the anti-tumor effects exerted by the fusion protein in HeLa-60 cells." in: Oncology letters, Vol. 9, Issue 6, pp. 2729-2735, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

    Zhang, Feng, Tao, DE, Chang, Hu: "Increased stathmin expression strengthens fear conditioning in epileptic rats." in: Biomedical reports, Vol. 3, Issue 1, pp. 28-32, 2015 (PubMed). Method employed by authors: Western Blotting (WB)

    Song, Xiao, Fu, Huang, Wang, Zhang, Yang: "Increased expression of the pluripotency markers sex-determining region Y-box 2 and Nanog homeobox in ovarian endometriosis." in: Reproductive biology and endocrinology : RB&E, Vol. 12, pp. 42, 2014 (PubMed). Method employed by authors: Western Blotting (WB) (1:10000, Sample species: Human).

    Yan, Yang, Li, Duan, Wu, Qian, Li, Wang: "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." in: Journal of cardiovascular pharmacology, Vol. 64, Issue 4, pp. 310-7, 2014 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

    Steenblock, Heckel, Czupalla, Espírito Santo, Niehage, Sztacho, Hoflack: "The Cdc42 guanine nucleotide exchange factor FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts." in: The Journal of biological chemistry, Vol. 289, Issue 26, pp. 18347-59, 2014 (PubMed). Method employed by authors: Western Blotting (WB)

    Li, McFadden, Morgan, Cui, Hu, Wan, Zeng: "All-trans retinoic acid regulates the expression of the extracellular matrix protein fibulin-1 in the guinea pig sclera and human scleral fibroblasts." in: Molecular vision, Vol. 16, pp. 689-97, 2010 (PubMed). Method employed by authors: Western Blotting (WB)

  • Target
    GAPDH
    Alternative Name
    Gapdh (GAPDH Antibody Abstract)
    Synonyms
    G3PD, GAPD, Gapd, BEST:GH12586, CG12055, Dmel\\CG12055, GA3PDH, GADPH, GAP, GAPDH, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, g3pd, G3PDH, glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase 1, glyceraldehyde-3-phosphate dehydrogenase S homeolog, glyceraldehyde-3-phosphate dehydrogenase, type I, olfactory receptor 8K3, GAPDH, Gapdh, Gapdh1, gapdh, gapdh.S, gapDH, LOC100404960, LOC614985
    Background

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

    Subcellular location: Cytoplasm

    Synonyms: G3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, CDABP47, OK/SW-cl.12

    Gene ID
    2597
    UniProt
    P04406
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