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Alpaca anti-Rabbit IgG Antibody (Alexa Fluor 568) - Preadsorbed Secondary Antibody

IF, SRM, WB Monoclonal VHH0244-VHH0245 Recombinant Antibody Alexa Fluor 568 single-domain Antibody (sdAb)
Catalog No. ABIN6810545
$405.00
Plus shipping costs $45.00
100 μL ABIN6810545
100 μL ABIN6810545
local_shipping Shipping to: United States
Delivery in 5 to 6 Business Days
  • Target
    IgG
    Antibody Type
    Recombinant Antibody
    Fragment
    single-domain Antibody (sdAb)
    Reactivity
    • 367
    • 333
    • 314
    • 224
    • 213
    • 111
    • 110
    • 82
    • 76
    • 73
    • 72
    • 65
    • 61
    • 56
    • 54
    • 49
    • 42
    • 31
    • 27
    • 15
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rabbit
    Host
    • 822
    • 781
    • 312
    • 178
    • 76
    • 75
    • 22
    • 20
    • 16
    • 12
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    Alpaca
    Clonality
    • 1787
    • 176
    Monoclonal
    Conjugate
    • 317
    • 233
    • 216
    • 207
    • 147
    • 146
    • 128
    • 72
    • 49
    • 46
    • 36
    • 31
    • 29
    • 28
    • 26
    • 26
    • 26
    • 25
    • 25
    • 25
    • 24
    • 23
    • 23
    • 23
    • 23
    • 23
    • 23
    • 22
    • 21
    • 19
    • 19
    • 18
    • 18
    • 14
    • 12
    • 11
    • 10
    • 9
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Alexa Fluor 568
    Application
    • 1261
    • 1111
    • 917
    • 806
    • 538
    • 525
    • 499
    • 144
    • 100
    • 71
    • 71
    • 65
    • 52
    • 50
    • 29
    • 28
    • 16
    • 13
    • 11
    • 10
    • 10
    • 9
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Super-resolution Microscopy (SRM), Western Blotting (WB)
    Purpose
    Alpaca single domain antibodies, monovalent
    Specificity
    This Nanobody mixture recognizes Fab and Fc fragments of rabbit IgG
    Cross-Reactivity (Details)
    Cross-reactivity to: Human and macaque serum
    No cross-reactivity to: Mouse, rat, sheep, goat, and guinea pig serum
    Characteristics
    Alpaca anti-rabbit IgG, recombinant VHH is an anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 anti-rabbit Nanobodies that bind to rabbit IgG with high affinity & specificity. The product belongs to the Nano-Secondaries family.
    Purification
    Preadsorption: During product development, we exclude Nanobodies that cross react to other commonly used species' IgGs.
    Immunogen
    Immunogen: Purified rabbit IgG.
    Alpaca-derived antibody, recombinantly produced in bacteria. Biclonal, mixture of 2 monoclonal Nanobodies.
    Clone
    VHH0244-VHH0245
    Affinity
    VHH0244: KD = 0.2 nM, VHH0245: KD = 1.2 nM
    Labeling Ratio
    Degree of labeling (DOL): 2 fluorophores per Nanobody
  • Application Notes
    Validation: Application validated for immunofluorescence, SRM, and Western blotting
    Determination of cross-reactivity, sequence, affinity, melting point, and degree of labeling (DOL)
    Fast one-step immunostaining: Simultaneous incubation of primary antibody and alpaca anti-rabbit IgG, recombinant VHH
    Immunofluorescence 1:1,000
    Super-resolution microscopy 1:1,000
    Western blot 1:1,000
    Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000.
    Note: Image acquisition time may have to be optimized.
    Comment

    Benefits:

    • Reduced incubation and hands-on time because the Alpaca anti-rabbit IgG, recombinant VHH can be incubated together with the rabbit primary antibody
    • Higher resolution imaging due to the Alpaca anti-rabbit IgG, recombinant VHHs' 10 times smaller size than conventional secondary antibodies. The small size enables better tissue penetration, antigen access and decreases the distance between the epitope and label.
    • Cleaner Images: The Alpaca anti-mouse IgG, recombinant VHH has a high specificity and low background:
      We select only VHHs with the desired specificity during development, VHHs that cross-react to other commonly used species' IgGs are eliminated. Hence, Nano-Secondaries have an extreme low background, any kind of pre-adsorption is not needed.
    • Multiplexing: the Alpaca anti-rabbit IgG, recombinant VHH does not cross-react to other frequently used IgGs and makes multiplexing possible.

    Restrictions
    For Research Use only
  • Concentration
    0.5 g/L
    Buffer
    10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,-20 °C,-80 °C
    Storage Comment

    Storage: -20 °C

    storageComment: Shipped at ambient temperature. Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. Stable for 6 months after arrival.

    Expiry Date
    6 months
  • Target
    IgG
    Background
    Next to conventional antibodies, alpacas possess also single domain antibodies (sdAbs). sdAbs contain only heavy chains and are devoid of light chains. The antigen binding domain of a sdAb is called VHH or Nanobody. Nano-Secondaries are IgG specific Nanobodies that are coupled to Alexa Fluor dyes.
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