For best experience we recommend to activate Javascript in your browser.

CD33 antibody (AA 101-200)

Name: CD33 antibody (AA 101-200)
SKU: ABIN687607
Price: 384.62 USD
Availability: InStock

CD33 Reactivity: Human, Mouse FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p), ICC Host: Rabbit Polyclonal unconjugated

Mouse splenocytes probed with CD33 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

FACS

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti CD33/Siglec-3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-CD33/Siglec-3 Polyclonal Antibody, Unconjugated (ABIN687607) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti CD33/Siglec-3 Polyclonal Antibody, Unconjugated (ABIN687607) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Lane 1: human colon carcinoma lysates Lane 2: rat lung lysates probed with Anti CD33 Polyclonal Antibody, Unconjugated (ABIN687607) at 1:200 in 4 °C.

SDS

6 images

(5 references)

Catalog No. ABIN687607

Datasheet as PDF

Target See all CD33 Antibodies

CD33
Binding Specificity
16

15

13

9

8

5

5

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 101-200
Reactivity
162

34

15

13

7

6

3

1
Human, Mouse
Host
94

74
Rabbit
Compatible Secondaries
Clonality
89

78

1
Polyclonal
Conjugate
57

15

13

12

9

5

5

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD33 antibody is un-conjugated
Application
103

101

38

30

25

25

23

22

15

12

10

9

6

4

4

2

2

2

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity

Human, Mouse

Predicted Reactivity

Rat

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse CD33

Isotype

IgG
anti-CD33 (CD33) (AA 15-237) antibody
CD33 Reactivity: Human FACS, WB, ELISA, IHC Host: Mouse Monoclonal 2C6B7 unconjugated

anti-CD33 (CD33) (AA 18-240) antibody
CD33 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 49-259) antibody
CD33 Reactivity: Human WB, IF, IHC Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 261-364) antibody
CD33 Reactivity: Human FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 1-364) antibody
CD33 Reactivity: Human WB, ELISA, RNAi Host: Mouse Monoclonal 2D12-G4 unconjugated

anti-CD33 (CD33) (AA 18-269) antibody
CD33 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM77 unconjugated

anti-CD33 (CD33) antibody
CD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, CyTOF Host: Mouse Monoclonal WM53 unconjugated

anti-CD33 (CD33) antibody
CD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, Func, CyTOF Host: Mouse Monoclonal WM53 unconjugated

anti-CD33 (CD33) antibody (Biotin)
CD33 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal HIM3-4 Biotin

anti-CD33 (CD33) antibody (FITC)
CD33 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal WM53 FITC

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Shan, Bu, Zhang, Zhang, Ding, Chang, Dai, Shen, Ma: "LukS-PV, a component of Panton-Valentine leukocidin, exerts potent activity against acute myeloid leukemia in vitro and in vivo." in: The international journal of biochemistry & cell biology, Vol. 61C, pp. 20-28, (2015) (PubMed).

Iwami, Brinkman, Bromberg: "Vascular endothelial growth factor c/vascular endothelial growth factor receptor 3 signaling regulates chemokine gradients and lymphocyte migration from tissues to lymphatics." in: Transplantation, Vol. 99, Issue 4, pp. 668-77, (2015) (PubMed).

Jiang, Li, Shi, Wu, An, Yang, Xu: "Involvement of p38 in signal switching from autophagy to apoptosis via the PERK/eIF2α/ATF4 axis in selenite-treated NB4 cells." in: Cell death & disease, Vol. 5, pp. e1270, (2015) (PubMed).

Shi, Jiang, Li, Shan, Li, An, Yang, Xu: "Sodium selenite alters microtubule assembly and induces apoptosis in vitro and in vivo." in: Journal of hematology & oncology, Vol. 6, Issue 1, pp. 7, (2013) (PubMed).
Target

CD33

Alternative Name

CD33 (CD33 Products)

Synonyms

SIGLEC-3 antibody, SIGLEC3 antibody, p67 antibody, Siglec-3 antibody, gp67 antibody, CD33 molecule antibody, CD33 antigen antibody, CD33 antibody, Cd33 antibody

Background

Synonyms: gp67, Siglec-3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, CD33, Siglec3
Background: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid (By similarity). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Gene ID

12489

UniProt

Q63994