CDC20 antibody
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- Target See all CDC20 Antibodies
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CDC20 antibody is un-conjugated
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Application
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human Cdc20 protein
- Clone
- CDC20-1102
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CDC20 Primary Antibody
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- Application Notes
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Known_Application: Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Ramos or HeLa cells. Tonsil or gastric carcinoma.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
- Alternative Name
- CDC20 (CDC20 Products)
- Synonyms
- X-FZY antibody, ba276h19.3 antibody, cdc20a antibody, fizzy1 antibody, p55cdc antibody, CDC20A antibody, bA276H19.3 antibody, p55CDC antibody, 2310042N09Rik antibody, C87100 antibody, cell division cycle 20 antibody, cell division cycle 20 L homeolog antibody, cell division cycle 20 homolog (S. cerevisiae) antibody, cdc20 antibody, CDC20 antibody, cdc20.L antibody, Cdc20 antibody
- Background
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CDC20, CDC20A, p55CDC, P55CDC-LSB,Cdc20 (Cell Division Cycle Protein 20)
Cellular localisation: Cytoplasmic - Molecular Weight
- 55kDa
- Gene ID
- 991, 524947
- UniProt
- Q12834
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