MAML3 antibody

Catalog No. ABIN6937803

Product Details anti-MAML3 Antibody

Target: MAML3 (Mastermind-Like 3 (MAML3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAML3 antibody is un-conjugated

Application: Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human MAML3 protein fragment

Clone: MAML3-1303

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MAML3

Target: MAML3 (Mastermind-Like 3 (MAML3))

Alternative Name: MAML3 (MAML3 Products)

Synonyms: AV234550 antibody, BC049812 antibody, Mam-2 antibody, mKIAA1816 antibody, Glrp1 antibody, CAGH3 antibody, ERDA3 antibody, GDN antibody, MAM-2 antibody, MAM2 antibody, TNRC3 antibody, mastermind like 3 (Drosophila) antibody, mastermind-like transcriptional coactivator 3 antibody, mastermind like transcriptional coactivator 3 antibody, Maml3 antibody, MAML3 antibody

Background: CAGH3, ERDA3, GDN, MAML3, Mastermind-like protein 3, TNRC3,MAML3 (Mastermind Like Transcriptional Coactivator 3)
Cellular localisation: Cytoplasmic

Molecular Weight: 150-170kDa

Gene ID: 55534, 586165

UniProt: Q96JK9