UACA antibody

Catalog No. ABIN6938715

Product Details anti-UACA Antibody

Target: UACA

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UACA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Specificity: UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.

Cross-Reactivity (Details): Human. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Nuclei of myeloid leukemia biopsy cells

Clone: AE-5

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or 293T cells. Highly expressed in skeletal muscle, heart, kidney and pancreas. Also expressed in epidermal melanocytes, eye muscles and thyroid follicular cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for UACA

Target: UACA

Alternative Name: UACA (UACA Products)

Synonyms: NUCLING antibody, 2700059D02Rik antibody, nucling antibody, RGD1560011 antibody, uveal autoantigen with coiled-coil domains and ankyrin repeats antibody, UACA antibody, Uaca antibody

Background: Nuclear membrane binding protein, Uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), NUCLING,UACA / Nucling (Nuclear Membrane Marker)
Cellular localisation: Nuclear membrane

Molecular Weight: 160kDa

Gene ID: 55075, 108049

Pathways: Positive Regulation of Endopeptidase Activity