Recombinant CFTR antibody (AA 258-385)

Catalog No. ABIN6939095

Product Details anti-CFTR Antibody

Target: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 258-385

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CFTR antibody is un-conjugated

Application: Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Cross-Reactivity (Details): Human,

Purification: 200ug/ml of Ab Purified by Protein A.

Immunogen: Recombinant fragment (around aa 258-385) of human CFTR protein (exact sequence is proprietary)

Clone: CFTR-1775R

Isotype: IgG

Application Details

Application Notes:

Positive Control: MOLT-4 cells. Pancreas, Kidney or Placenta.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for CFTR

Target: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Alternative Name: CFTR (CFTR Products)

Synonyms: ABC35 antibody, ABCC7 antibody, CF antibody, CFTR/MRP antibody, MRP7 antibody, TNR-CFTR antibody, dJ760C5.1 antibody, CFTR antibody, AW495489 antibody, Abcc7 antibody, RGD1561193 antibody, abc35 antibody, abcc7 antibody, cftr/mrp antibody, mrp7 antibody, tnr-cftr antibody, xcftr antibody, si:dkey-270i2.2 antibody, cystic fibrosis transmembrane conductance regulator antibody, cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) antibody, CFTR antibody, cftr-A antibody, Cftr antibody, cftr antibody

Background: ABC35, ATP Binding Cassette Superfamily C Member 7 (ABCC7), cAMP-dependent chloride channel, CFTR, CFTR/MRP, Channel conductance-controlling ATPase, Cystic Fibrosis Transmembrane Conductance Regulator, MRP7, TNR CFTR,CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: 165-170kDa

Gene ID: 1080, 489786, 621460

UniProt: P13569