CGA antibody

Catalog No. ABIN6939100

Product Details anti-CGA Antibody

Target: CGA (Glycoprotein Hormones, alpha Polypeptide (CGA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CGA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM), Immunostaining (ISt)

Specificity: This MAb reacts with a protein of ~13 kDa, identified as alpha sub-unit of Luteinizing Hormone (LH). Its structure is similar to the other glycoproteins, follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG). The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits that are connected by two bridges. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. LH has a beta subunit of 121 amino acids (LHB) that confers its specific biologic action and is responsible for interaction with the LH receptor. This beta subunit contains the same amino acids in sequence as the beta subunit of hCG and both stimulate the same receptor, however, the hCG beta subunit contains an additional 24 amino acids and the hormones differ in the composition of their sugar moieties.LH is synthesized and secreted by gonadotrophs in the anterior lobe of the pituitary gland. In concert with the other pituitary gonadotropin follicle-stimulating hormone (FSH), it is necessary for proper reproductive function. In the female, an acute rise of LH levels triggers ovulation. In the male, where LH has also been called Interstitial Cell-Stimulating Hormone (ICSH), it stimulates Leydig cell production of testosterone. LH is a useful marker in classification of pituitary tumors and the study of pituitary disease.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length hLH alpha protein

Clone: LHa-756

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Anterior Pituitary.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(No special pre-treatment is required for staining of formalin-fixed tissues. Note that HIER is NOT recommended for this antibody.)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CGA

Target: CGA (Glycoprotein Hormones, alpha Polypeptide (CGA))

Alternative Name: LHA (CGA Products)

Synonyms: CG-ALPHA antibody, FSHA antibody, GPHA1 antibody, GPHa antibody, HCG antibody, LHA antibody, TSHA antibody, CG-alpha antibody, GPHalpha antibody, Tsha antibody, aGSU antibody, alpha-GSU antibody, alphaGSU antibody, alphaSU antibody, CGA antibody, FSH-alpha antibody, LSH-alpha antibody, TSH-alpha antibody, cg-alpha antibody, cga antibody, cga-a antibody, cga-b antibody, fsha antibody, gpha antibody, gpha1 antibody, hcg antibody, lha antibody, tsha antibody, GTH-alpha antibody, gsu-alpha antibody, glycoprotein hormones, alpha polypeptide antibody, glycoprotein hormones, alpha subunit antibody, chromogranin A antibody, glycoprotein hormones, alpha polypeptide L homeolog antibody, CGA antibody, Cga antibody, CHGA antibody, cga.L antibody, cga antibody

Molecular Weight: ~13kDa

Gene ID: 1081

UniProt: P01215

Pathways: Metabolism of Steroid Hormones and Vitamin D, Thyroid Hormone Synthesis, Hormone Transport, Peptide Hormone Metabolism