COL7A1 antibody
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- Target See all COL7A1 Antibodies
- COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))
- Reactivity
- Human, Cow, Goat, Guinea Pig, Horse, Sheep
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This COL7A1 antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Staining Methods (StM)
- Specificity
- Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant full-length human Collagen VII protein
- Clone
- LH7-2
- Isotype
- IgG2a kappa
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- Application Notes
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Positive Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.
Known Application: ELISA (For coating, order antibody without BSA), Western Blot (0.5-1.0 μg/mL)Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))
- Alternative Name
- COL7A1 (COL7A1 Products)
- Synonyms
- COL7A1 antibody, EBD1 antibody, EBDCT antibody, EBR1 antibody, AW209154 antibody, collagen type VII alpha 1 chain antibody, collagen, type VII, alpha 1 antibody, COL7A1 antibody, col7a1 antibody, Col7a1 antibody
- Molecular Weight
- 290kDa
- Gene ID
- 1294
- UniProt
- Q02388
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