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COL7A1 antibody

COL7A1 Reactivity: Human, Cow, Goat, Guinea Pig, Horse, Sheep ELISA, WB, IHC, IF, Coat, FACS, StM Host: Mouse Monoclonal LH7-2 unconjugated
Catalog No. ABIN6939168
  • Target See all COL7A1 Antibodies
    COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))
    Reactivity
    Human, Cow, Goat, Guinea Pig, Horse, Sheep
    Host
    • 23
    • 1
    • 1
    Mouse
    Clonality
    • 24
    • 1
    Monoclonal
    Conjugate
    • 10
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This COL7A1 antibody is un-conjugated
    Application
    • 9
    • 6
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Staining Methods (StM)
    Specificity
    Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant full-length human Collagen VII protein
    Clone
    LH7-2
    Isotype
    IgG2a kappa
  • Application Notes

    Positive Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.

    Known Application: ELISA (For coating, order antibody without BSA), Western Blot (0.5-1.0 μg/mL)Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))
    Alternative Name
    COL7A1 (COL7A1 Products)
    Synonyms
    COL7A1 antibody, EBD1 antibody, EBDCT antibody, EBR1 antibody, AW209154 antibody, collagen type VII alpha 1 chain antibody, collagen, type VII, alpha 1 antibody, COL7A1 antibody, col7a1 antibody, Col7a1 antibody
    Molecular Weight
    290kDa
    Gene ID
    1294
    UniProt
    Q02388
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