Recombinant CD21 antibody (AA 142-240)

Catalog No. ABIN6939186

Product Details anti-CD21 Antibody

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 142-240

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD21 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Staining Methods (StM)

Specificity: Recognizes a protein of 140 kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 142-240) of human CR2 (CD21) protein (exact sequence is proprietary)

Clone: CR2-3124R

Isotype: IgG

Application Details

Application Notes:

Positive Control: MOLT4, WEHI-231 cells and Tonsil.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CR2 (CR2 Products)

Synonyms: C3DR antibody, CD21 antibody, CR antibody, CVID7 antibody, SLEB9 antibody, Cd21 antibody, cd21 antibody, CR1 antibody, CR1-L antibody, CR2 antibody, complement C3d receptor 2 antibody, complement C3b/C4b receptor 1 (Knops blood group) antibody, CR2 antibody, Cr2 antibody, CR1 antibody

Molecular Weight: 140kDa

Gene ID: 1380

UniProt: P20023

Pathways: Complement System