XRCC6/XRCC5 antibody

Catalog No. ABIN6939480

Product Details anti-XRCC6/XRCC5 Antibody

Target: XRCC6/XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This XRCC6/XRCC5 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity: Recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

No Cross-Reactivity: Chicken, Mouse (Murine), Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Nuclear extract of human HL-60 cells

Clone: KU729

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Human cancer K562, HL-60, HEp-2 or HeLa cells.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunocytochemistry (Acetone-fixed cells) (0.5-1.0 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for XRCC6/XRCC5

Target: XRCC6/XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5))

Alternative Name: XRCC6 (p70) & XRCC5 (p80) (XRCC6/XRCC5 Products)

Molecular Weight: 70kDa & 80kDa

Gene ID: 2547, 7520

UniProt: P12956, P13010