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IGKC antibody

IGKC Reactivity: Human IHC, FACS, WB, StM Host: Mouse Monoclonal KLC264 unconjugated
Catalog No. ABIN6934397
  • Target See all IGKC products
    IGKC (Immunoglobulin kappa Constant (IGKC))
    Reactivity
    • 30
    • 2
    Human
    Host
    • 18
    • 14
    Mouse
    Clonality
    • 24
    • 8
    Monoclonal
    Conjugate
    • 25
    • 3
    • 2
    • 1
    • 1
    This IGKC antibody is un-conjugated
    Application
    • 12
    • 12
    • 12
    • 11
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 1
    Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), Staining Methods (StM)
    Specificity
    This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant human Ig kappa chain
    Clone
    KLC264
    Isotype
    IgG1 kappa
  • Application Notes

    Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

    Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    IGKC (Immunoglobulin kappa Constant (IGKC))
    Alternative Name
    IGKC (IGKC Products)
    Synonyms
    HCAK1 antibody, IGKCD antibody, Km antibody, IGKC antibody, Igk-C antibody, immunoglobulin kappa constant antibody, immunoglobulin kappa constant L homeolog antibody, IGKC antibody, LOC708048 antibody, igkc antibody, igkc.L antibody, Igkc antibody
    Molecular Weight
    ~22.5kDa
    Gene ID
    3514
    UniProt
    P01601, P01834
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