NPM1 antibody (AA 185-287)

Catalog No. ABIN6940221

Product Details anti-NPM1 Antibody

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Binding Specificity: AA 185-287

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Specificity: Recognizes a 33 kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm.It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.Mutations in this gene are associated with acute myeloid leukemia.The antibody may be a useful aid for classification of acute myeloid leukemia.

Purification: Purified by Protein A/G

Immunogen: Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)

Clone: NPM1-3398

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: MCF-7, HeLa or A431 cells. Skin, Colon.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM1 (NPM1 Products)

Synonyms: B23 antibody, NPM antibody, NO38 antibody, Npm antibody, B23NP antibody, npm4 antibody, npm antibody, CG7917 antibody, CRP1 antibody, Crp1 antibody, Dmel\\CG7917 antibody, dNLP antibody, dNLP-S antibody, p22 antibody, npm1 antibody, sb:cb487 antibody, nucleophosmin 1 antibody, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog antibody, nucleophosmin 1b antibody, Nucleophosmin antibody, nucleophosmin antibody, nucleophosmin (nucleolar phosphoprotein B23, numatrin) antibody, Nucleoplasmin antibody, nucleophosmin 1a antibody, NPM1 antibody, Npm1 antibody, npm1.L antibody, npm1b antibody, npm antibody, Nlp antibody, npm1a antibody

Molecular Weight: 33kDa

Gene ID: 4869

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly