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Recombinant CD45 antibody

PTPRC Reactivity: Human FACS, IHC, WB, IF, StM Host: Mouse Monoclonal rPTPRC-1460 unconjugated Recombinant Antibody
Catalog No. ABIN6940452
  • Target See all CD45 (PTPRC) Antibodies
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 393
    • 227
    • 79
    • 29
    • 20
    • 16
    • 15
    • 10
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 281
    • 178
    • 148
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 466
    • 137
    • 1
    Monoclonal
    Conjugate
    • 174
    • 60
    • 51
    • 48
    • 39
    • 15
    • 13
    • 11
    • 10
    • 10
    • 9
    • 9
    • 8
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD45 antibody is un-conjugated
    Application
    • 502
    • 206
    • 151
    • 126
    • 126
    • 74
    • 68
    • 58
    • 47
    • 27
    • 17
    • 14
    • 14
    • 13
    • 12
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Staining Methods (StM)
    Purification
    Purified by Protein A/G
    Immunogen
    Human recombinant full-length CD45 protein
    Clone
    RPTPRC-1460
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product PTPRC Primary Antibody
  • Application Notes

    Positive Control: Ramos, U-698 or GA-10 cells. Tonsil and Spleen.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    Alternative Name
    PTPRC (PTPRC Products)
    Synonyms
    PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody
    Background
    CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
    Molecular Weight
    180-220kDa
    Gene ID
    5788
    UniProt
    P08575
    Pathways
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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