Smooth Muscle Actin antibody
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- Target See all Smooth Muscle Actin (ACTA2) Antibodies
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
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Reactivity
- Human, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Smooth Muscle Actin antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- Specificity
- Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
- Cross-Reactivity (Details)
- Predicted to show a broad reactivity.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant full-length human ACTA2 protein
- Clone
- ACTA2-791
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product ACTA2 Primary Antibody
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- Application Notes
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Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
- Alternative Name
- ACTA2 (ACTA2 Products)
- Synonyms
- AAT6 antibody, ACTSA antibody, MYMY5 antibody, GAMMA-ACTIN antibody, ACT antibody, ACTA3 antibody, ACTE antibody, ACTL3 antibody, ACTSG antibody, Act-4 antibody, Act4 antibody, SMGA antibody, 0610041G09Rik antibody, Actvs antibody, SMalphaA antibody, a-SMA antibody, alphaSMA antibody, wu:fb63d03 antibody, ASMA antibody, SMactin antibody, a-sma antibody, aat6 antibody, actsa antibody, mymy5 antibody, sm-actin antibody, ACTA2 antibody, actc1 antibody, ACT-4 antibody, asma antibody, ACTGE antibody, actin, alpha 2, smooth muscle, aorta antibody, actin, gamma 2, smooth muscle, enteric antibody, actin, alpha 2, smooth muscle, aorta L homeolog antibody, ACTA2 antibody, ACTG2 antibody, Actg2 antibody, Acta2 antibody, acta2 antibody, acta2.L antibody
- Molecular Weight
- 42kDa
- Gene ID
- 59
- UniProt
- P62736
- Pathways
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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