S100A1 antibody

Catalog No. ABIN6940539

Product Details anti-S100A1 Antibody

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length S100A1 protein

Clone: S100A1-1942

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: A431 or HT-29 cells. Brain or Melanoma.

Known Application: Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Alternative Name: S100A1 (S100A1 Products)

Synonyms: S100 antibody, S100-alpha antibody, S100A antibody, S100A1 antibody, DKFZp468K0511 antibody, AI266795 antibody, S100a antibody, S100 calcium binding protein A1 antibody, S100A1 antibody, S100a1 antibody, s100a1 antibody

Molecular Weight: 10-12kDa

Gene ID: 6271

UniProt: P23297

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins