S100A9 antibody

Catalog No. ABIN6940550

Product Details anti-S100A9 Antibody

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A9 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: Recognizes a 14 kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14), expressed by granulocytes, monocytes and by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).

Immunogen: Recombinant human S100A9 protein

Clone: S100A9-1011

Isotype: IgM kappa

Application Details

Application Notes:

Positive Control: Liver or histiocytoma.

Known Application: Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100A9 (S100A9 Products)

Synonyms: 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, S100A9 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody

Molecular Weight: 14kDa

Gene ID: 6280

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins