SOX10 antibody (AA 115-269)

Catalog No. ABIN6940625

Product Details anti-SOX10 Antibody

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Binding Specificity: AA 115-269

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately-to-strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Purification: Purified by Protein A/G

Immunogen: Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary)

Clone: SOX10-1074

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: HepG2 cells. Melanomas, breast carcinomas, gliomas.

Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Alternative Name: SOX10 (SOX10 Products)

Synonyms: DOM antibody, PCWH antibody, WS2E antibody, WS4 antibody, WS4C antibody, SOX-10 antibody, SOX10 antibody, dom antibody, ws4 antibody, Dom antibody, Sox21 antibody, SOX9 antibody, cls/sox10 antibody, zgc:100757 antibody, SRY-box 10 antibody, SRY box 10 antibody, SRY-box 10 L homeolog antibody, SRY (sex determining region Y)-box 10 antibody, SOX10 antibody, Sox10 antibody, sox10.L antibody, sox10 antibody

Molecular Weight: 49-58kDa

Gene ID: 6663

UniProt: P56693

Pathways: Chromatin Binding