Transferrin Receptor antibody (AA 94-212)

Catalog No. ABIN6940700

Product Details anti-Transferrin Receptor Antibody

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Binding Specificity: AA 94-212

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Specificity: It recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Purification: Purified by Protein A/G

Immunogen: Recombinant extracellular fragment (around aa 94-212) of human TFRC protein (exact sequence is proprietary)

Clone: TFRC-1818

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: Jurkat, HeLa, MCF-7 or K562 cells. Human placenta or Bone Marrow.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: TFRC (TFRC Products)

Synonyms: Trfr antibody, LOC100219280 antibody, tfr antibody, cd71 antibody, tfr1 antibody, trfr antibody, CD71 antibody, T9 antibody, TFR antibody, TFR1 antibody, TR antibody, TRFR antibody, p90 antibody, 2610028K12Rik antibody, AI195355 antibody, AI426448 antibody, AU015758 antibody, E430033M20Rik antibody, Mtvr-1 antibody, Mtvr1 antibody, transferrin receptor antibody, TFRC antibody, Tfrc antibody, tfrc antibody

Molecular Weight: 85-95kDa (monomer), 190kDa (dimer)

Gene ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis