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TNF alpha antibody

TNF alpha Reactivity: Human FACS, IF, CyTox Host: Mouse Monoclonal J2D10 unconjugated
Catalog No. ABIN6940758
  • Target See all TNF alpha Antibodies
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Reactivity
    • 323
    • 182
    • 123
    • 67
    • 50
    • 42
    • 27
    • 26
    • 23
    • 16
    • 12
    • 10
    • 10
    • 9
    • 6
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 262
    • 192
    • 31
    • 25
    • 6
    • 2
    • 2
    Mouse
    Clonality
    • 278
    • 233
    Monoclonal
    Conjugate
    • 248
    • 86
    • 45
    • 20
    • 17
    • 12
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This TNF alpha antibody is un-conjugated
    Application
    • 348
    • 210
    • 129
    • 121
    • 74
    • 61
    • 58
    • 49
    • 48
    • 40
    • 39
    • 38
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    • 32
    • 20
    • 12
    • 10
    • 9
    • 9
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Cytotoxicity Test (CyTox)
    No Cross-Reactivity
    Mouse (Murine)
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant human TNF-alpha
    Clone
    J2D10
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product TNF alpha Primary Antibody
  • Application Notes

    Positive Control: HeLa, HL-60 or A431 cells. Macrophages in lymph node or Tonsil.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Inhibits Tumor Growth (Order Ab without BSA/Azide), Neutralizes rTNF- Mediated Cytotoxicity (Order Ab without BSA/Azide), Protects Mice Against Toxicity of rhTNF- (Order Ab without BSA/Azide)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Alternative Name
    TNF (TNF alpha Products)
    Synonyms
    DIF antibody, TNF-alpha antibody, TNFA antibody, TNFSF2 antibody, RATTNF antibody, Tnfa antibody, tnf antibody, TNF-a antibody, TNFalpha antibody, Tnfsf1a antibody, TNFa antibody, cTNF antibody, Tnf-alpha antibody, tnfa-like antibody, TNF-ALPHA antibody, dif antibody, tnfa antibody, xtnf antibody, tnfsf2 antibody, tnf-alpha antibody, Cachectin antibody, tumor necrosis factor antibody, tumor necrosis factor b (TNF superfamily, member 2) antibody, tumor necrosis factor alpha antibody, tumor necrosis factor a (TNF superfamily, member 2) antibody, TNF antibody, Tnf antibody, tnf antibody, tnfb antibody, tnf-alpha antibody, LOC103694380 antibody, tnfa antibody
    Background
    This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
    Molecular Weight
    17kDa
    Gene ID
    7124
    UniProt
    P01375
    Pathways
    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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