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CD14 antibody (AA 25-148)

Name: CD14 antibody (AA 25-148)
Brand: antibodies-online
SKU: ABIN6941096
Price: 597.38 USD
Availability: InStock

CD14 Reactivity: Human WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2408 unconjugated

Western Blot Analysis of A549 cell lysate using CD14 Mouse Monoclonal Antibody (LPSR/2408).

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD14 Mouse Monoclonal Antibody (LPSR/2408) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array.

PAr

Formalin-fixed, paraffin-embedded human Lymph Node stained with CD14 Mouse Monoclonal Antibody (LPSR/2408).

IHC

Formalin-fixed, paraffin-embedded human Tonsil stained with CD14 Mouse Monoclonal Antibody (LPSR/2408).

IHC

SDS-PAGE Analysis Purified CD14 Mouse Monoclonal Antibody (LPSR/2408).

SDS

5 images

Catalog No. ABIN6941096

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Binding Specificity
16

9

7

6

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 25-148
Reactivity
246

47

30

29

28

28

25

24

22

18

15

14

10

9

8

8

8

5

2

2

2

1

1

1
Human
Host
204

63

16

3

1
Mouse
Compatible Secondaries
Clonality
223

64
Monoclonal
Conjugate
120

29

22

17

13

6

5

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
Application
202

98

61

52

46

44

22

21

20

14

13

13

7

4

4

3

3

3

3

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Specificity

Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Purification

Purified by Protein A/G

Immunogen

Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)

Clone

LPSR-2408

Isotype

IgG1 kappa

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Application Notes

Positive Control: A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.
Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions

For Research Use only
Concentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date

24 months
Target

CD14

Alternative Name

CD14 (CD14 Products)

Synonyms

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Molecular Weight

55kDa

Gene ID

929

UniProt

P08571

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades