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BrdU antibody

BrdU IHC, Neut, StM Host: Mouse Monoclonal BRD469 unconjugated
Catalog No. ABIN6941340
  • Target See all BrdU Antibodies
    BrdU (Bromodeoxyuridine (BrdU))
    Reactivity
    Please inquire
    Host
    • 36
    • 22
    • 6
    • 2
    • 1
    Mouse
    Clonality
    • 44
    • 23
    Monoclonal
    Conjugate
    • 31
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This BrdU antibody is un-conjugated
    Application
    • 26
    • 26
    • 23
    • 22
    • 18
    • 13
    • 13
    • 12
    • 9
    • 6
    • 6
    • 6
    • 5
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Neutralization (Neut), Staining Methods (StM)
    Specificity
    It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
    Purification
    Purified by Protein A/G
    Immunogen
    Bromodeoxyuridine (BrdU) conjugated to KLH
    Clone
    BRD469
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product BrdU Primary Antibody
  • Application Notes

    Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

    Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Use Ab at 1 μg/mL for 30 minute at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC (Cat. #AP-9008)]Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    BrdU (Bromodeoxyuridine (BrdU))
    Alternative Name
    Bromodeoxyuridine (BrdU) (Proliferation Marker) (BrdU Products)
    Target Type
    Chemical
    Molecular Weight
    307Da
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