Piwi-Like 1 (Drosophila) (PIWIL1) antibody

Details for Product No. ABIN6944551
Antigen
  • ziwi
  • PIWI
  • HIWI
  • MIWI
  • piwi like RNA-mediated gene silencing 1
  • PIWI-like protein 1
  • putative PIWI-like protein 1
  • putative argonaut-like protein
  • piwi-like RNA-mediated gene silencing 1
  • PIWIL1
  • Tb10.70.5520
  • LINJ_21_0470
  • LBRM_21_0470
  • piwil1
  • Piwil1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
89
75
63
5
4
4
4
4
2
2
1
Host
Rabbit
95
8
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
5
4
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
53
37
36
13
10
9
6
3
2
1
1
1
Options
Immunogen Recombinant protein within human PIWIL1 aa 700-850
Clone 12C7
Isotype IgG
Purification Purified by Protein A.
Antigen
Alternative Name PIWIL1 (PIWIL1 Antibody Abstract)
Background

Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis, RNF8 being released upon ubiquitination and degradation of PIWIL1.

Subcellular location: Cytoplasm


Synonyms: Piwi-like protein 1, PIWIL1, HIWI., MIWI, CT80.1.
Gene ID 9271
UniProt Q96J94
Application Notes WB(1:500-1000), IHC-P(1:50-200)
Restrictions For Research Use only
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Expiry Date 12 months
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