SARS-CoV-2 Spike antibody

Catalog No. ABIN7072835

Product Details anti-SARS-CoV-2 Spike Antibody

Target: SARS-CoV-2 Spike

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SARS-CoV-2 Spike antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Characteristics: 2019-nCoV CoV spike/S Has cross-reactivity in ELISA with SARS Coronavirus Spike Protein (S1 Subunit), SARS Coronavirus Spike RBD

Application Details

Application Notes: ELISA 1:5000-1:10000
Validated Applications:ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)

Restrictions: For Research Use only

Handling

Buffer: 0.2 um filtered solution in PBS

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Target Details for SARS-CoV-2 Spike

Target: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Products

Synonyms: E2 antibody, E2 glycoprotein precursor antibody, Surface Glycoprotein antibody, S antibody

Target Type: Viral Protein

Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Gene ID: 43740568

UniProt: P0DTC2