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H2AFZ antibody (C-Term)

H2AFZ Reactivity: Human ChIP-seq, ChIP, CUT&RUN, CUT&Tag, DB, IF, WB Host: Rabbit Polyclonal unconjugated
Pubmed (1 reference)
Catalog No. ABIN6971770
$616.85
Plus shipping costs $45.00
100 μL
Shipping to: United States
Delivery in 5 to 7 Business Days
  • Target See all H2AFZ Antibodies
    H2AFZ (H2A Histone Family, Member Z (H2AFZ))
    Binding Specificity
    • 15
    • 15
    • 10
    • 9
    • 9
    • 8
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 95
    • 34
    • 32
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 98
    • 2
    Rabbit
    Clonality
    • 81
    • 19
    Polyclonal
    Conjugate
    • 59
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This H2AFZ antibody is un-conjugated
    Application
    • 61
    • 47
    • 31
    • 16
    • 14
    • 13
    • 13
    • 11
    • 9
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Dot Blot (DB), Immunofluorescence (IF), Western Blotting (WB)
    Characteristics
    The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may enable H2A.Z to function as an insulator of chromatin domains. Histone H2A.Z antibody (pAb) (H2AZ) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
    Purification
    Serum
    Immunogen
    This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • Akdogan-Ozdilek, Duval, Meng, Murphy, Goll: "Identification of chromatin states during zebrafish gastrulation using CUT&RUN and CUT&Tag." in: Developmental dynamics : an official publication of the American Association of Anatomists, (2021) (PubMed).

  • Target
    H2AFZ (H2A Histone Family, Member Z (H2AFZ))
    Alternative Name
    Histone H2A.Z (H2AFZ Products)
    Synonyms
    H2A.Z-1, H2A.z, H2A/z, H2AZ, H2A.Z, H2a.z-1, H2AFV, H2Az, XH2AZ, h2a.zl1, h2a.zl2, H2A histone family member Z, H2A histone family, member Z, H2A histone family member Z L homeolog, histone H2A.Z, H2AFZ, H2afz, h2afz.L
    Background
    The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may enable H2A.Z to function as an insulator of chromatin domains.
    Molecular Weight
    14 kDa
    NCBI Accession
    NP_002097
    Pathways
    Telomere Maintenance
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