SMARCA4 antibody (AA 213-295)

Catalog No. ABIN6972735

Product Details anti-SMARCA4 Antibody

Target: SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))

Binding Specificity: AA 213-295

Reactivity: Human, Mouse, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This SMARCA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Characteristics: SMARCA4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4) is the central catalytic ATPase subunit of numerous chromatin-remodeling complexes, including SWI/SNF. These complexes are able to use energy from ATP hydrolysis to physically disrupt chromatin architecture of target promoters and facilitate the binding of transcription factors to nucleosomal DNA. SMARCA4 is a co-regulator of transcription and has been implicated in the activation and repression of gene expression through the modulation of chromatin in various tissues and physiological conditions. This protein plays a major role in many cellular processes such as DNA replication, repair and recombination. Mammalian SMARCA4 is usually associated with approximately 10-12 BAF subunits or other proteins involved in regulation of gene expression. SMARCA4 is part of the stem cell-specific BAF complex, esBAF, and is involved in regulating stem cell pluripotency. SMARCA4 antibody (mAb) (Clone 5B7) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human, Monkey and Mouse samples.

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a recombinant protein corresponding to amino acids 213-295 of human SMARCA4.

Clone: 5B7

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for SMARCA4

Target: SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))

Alternative Name: SMARCA4 (SMARCA4 Products)

Synonyms: SMARCA4 antibody, BAF190 antibody, BAF190A antibody, BRG1 antibody, MRD16 antibody, RTPS2 antibody, SNF2 antibody, SNF2L4 antibody, SNF2LB antibody, SWI2 antibody, hSNF2b antibody, brg-1 antibody, Brg1 antibody, HP1-BP72 antibody, SNF2beta antibody, SW1/SNF antibody, b2b692Clo antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 antibody, SMARCA4 antibody, Smarca4 antibody

Molecular Weight: 180 kDa

NCBI Accession: NP_001122321

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance