SKA1 Antibody is affinity chromatography purified via peptide column.
Immunogen
SKA1 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA1. The immunogen is located within amino acids 180 - 230 of SKA1.
SKA1
Reactivity: Human
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Cy5
Application Notes
SKA1 antibody can be used for detection of SKA1 by Western blot at 0.5 - 1 μ,g/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SKA1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
SKA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
SKA1
(Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))
SKA1 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.