PLEKHM3 antibody (C-Term)

Catalog No. ABIN6991226

Product Details anti-PLEKHM3 Antibody

Target: PLEKHM3 (Pleckstrin Homology Domain Containing, Family M, Member 3 (PLEKHM3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLEKHM3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: PLEKHM3 Antibody is affinity chromatography purified via peptide column.

Immunogen: PLEKHM3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human PLEKHM3. The immunogen is located within the last 50 amino acids of PLEKHM3.

Isotype: IgG

Application Details

Application Notes: PLEKHM3 antibody can be used for detection of PLEKHM3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PLEKHM3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PLEKHM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PLEKHM3

Target: PLEKHM3 (Pleckstrin Homology Domain Containing, Family M, Member 3 (PLEKHM3))

Alternative Name: PLEKHM3 (PLEKHM3 Products)

Synonyms: PLEKHM1L antibody, DAPR antibody, 9430067K14Rik antibody, A230102O09Rik antibody, AI449570 antibody, Plekhm1l antibody, Dapr antibody, RGD1307151 antibody, pleckstrin homology domain containing, family M, member 3 antibody, pleckstrin homology domain containing M3 antibody, pleckstrin homology domain containing M3 L homeolog antibody, PLEKHM3 antibody, Plekhm3 antibody, plekhm3.L antibody

Background: PLEKHM3 Antibody: PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.

Gene ID: 389072

UniProt: Q6ZWE6