TRIM71 antibody (C-Term)

Catalog No. ABIN6991391

Product Details anti-TRIM71 Antibody

Target: TRIM71 (Tripartite Motif Containing 71, E3 Ubiquitin Protein Ligase (TRIM71))

Binding Specificity: AA 680-730, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM71 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: TRIM71 Antibody is affinity chromatography purified via peptide column.

Immunogen: TRIM71 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71. The immunogen is located within amino acids 680 - 730 of TRIM71.

Isotype: IgG

Application Details

Application Notes: TRIM71 antibody can be used for detection of TRIM71 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TRIM71 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TRIM71 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TRIM71

Target: TRIM71 (Tripartite Motif Containing 71, E3 Ubiquitin Protein Ligase (TRIM71))

Alternative Name: TRIM71 (TRIM71 Products)

Synonyms: LIN-41 antibody, LIN41 antibody, RGD1566388 antibody, 2610206G21Rik antibody, AL022943 antibody, Gm1127 antibody, Lin41 antibody, Ripply2 antibody, lin-41 antibody, mLin41 antibody, mlin-41 antibody, tripartite motif containing 71 antibody, tripartite motif-containing 71 antibody, TRIM71 antibody, Trim71 antibody

Background: TRIM71 Antibody: TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.

Gene ID: 131405

NCBI Accession: NP_001034200

UniProt: Q2Q1W2

Pathways: Tube Formation