Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
ATAD3B antibody is predicted to not cross-react with other ATAD protein family members. At least three isoforms of ATAD3B are known to exist.
Purification
ATAD3B Antibody is affinity chromatography purified via peptide column.
Immunogen
ATAD3B antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human ATAD3B. The immunogen is located within amino acids 570 - 620 of ATAD3B.
ATAD3B
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ATAD3B antibody can be used for detection of ATAD3B by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
ATAD3B Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
ATAD3B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ATAD3B Antibody: ATAD3B is a member of the AAA ATPase family, a family of proteins that catalyze ATP into ADP and are involved in several cellular functions such as cell-cycle regulation, protein proteolysis and transport. The AAA ATPase family is characterized by a highly conserved AAA motif containing Walker homology sequences and imparting ATPase activity. Mitochondrial membrane proteins ATAD3A/B contribute to the stabilization of nucleoids and may participate in the transformation pathway and the chemosensitivity of oligodendrogliomas. The gene encoding ATAD3A/B/C maps to human chromosome 1 and has been suggested to be an anti-apoptotic marker.