RC3H1 antibody (C-Term)

Catalog No. ABIN6991828

Product Details anti-RC3H1 Antibody

Target: RC3H1 (Ring Finger and CCCH-Type Domains 1 (RC3H1))

Binding Specificity: AA 1040-1090, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RC3H1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: At least three isoforms of RC3H1 are known to exist, this antibody will detect all three isoforms.

Purification: RC3H1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Rabbit polyclonal RC3H1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RC3H1. The immunogen is located within amino acids 1040 - 1090 of RC3H1.

Isotype: IgG

Application Details

Application Notes: RC3H1 antibody can be used for detection of RC3H1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RC3H1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RC3H1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for RC3H1

Target: RC3H1 (Ring Finger and CCCH-Type Domains 1 (RC3H1))

Alternative Name: RC3H1 (RC3H1 Products)

Background: RC3H1 Antibody: The ring finger protein RC3H1, also known as Roquin, is a highly conserved member of the RING type ubiquitin ligase protein family whose M199R mutation leads to the excessive production of follicular helper T cells and germinal centers in the sanroque strain of mice, a strain with excessive IL-21 production and high titers of autoantibodies. The complete loss of RC3H1 induces early death and immune deregulation but not autoimmunity in RC3H1-null mice, suggesting that the mutant RC3H1 is more disruptive to the immune system than its complete loss.

Molecular Weight: 125 kDa

Gene ID: 149041

NCBI Accession: NP_742068

UniProt: Q5TC82

Pathways: Ribonucleoprotein Complex Subunit Organization, Activated T Cell Proliferation