WNT5B antibody (AA 130-180)

Catalog No. ABIN6992093

Product Details anti-WNT5B Antibody

Target: WNT5B (Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B))

Binding Specificity: AA 130-180

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT5B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: WNT5 antibody is human, mouse and rat reactive. WNT5 antibody will detect both WNT5A and WNT5B. At least two isoforms of WNT5B are known to exist, this antibody will detect both isoforms.

Purification: WNT5 antibody is affinity chromatography purified via peptide column.

Immunogen: WNT5 antibody was raised against a 15 amino acid peptide near the center of human WNT5B. The immunogen is located within amino acids 130 - 180 of WNT5.

Isotype: IgG

Application Details

Application Notes: WNT5 antibody can be used for detection of WNT5 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: WNT5 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: WNT5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for WNT5B

Target: WNT5B (Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B))

Alternative Name: WNT5 (WNT5B Products)

Synonyms: wnt-5b antibody, xwnt5b antibody, WNT5B antibody, AW545702 antibody, Wnt-5b antibody, xwnt-5c antibody, CHUNP6928 antibody, id:ibd5111 antibody, ppt antibody, wnt-5 antibody, wnt5 antibody, wnt[b] antibody, wu:fk85g06 antibody, Wnt family member 5B antibody, wingless-type MMTV integration site family, member 5B antibody, Wnt family member 5B S homeolog antibody, wingless-type MMTV integration site family, member 5b antibody, wnt5b antibody, WNT5B antibody, Wnt5b antibody, wnt5b.S antibody

Background: WNT5A and WNT5B are near-identical members of the WNT gene family, which consists of structurally related genes which encode secreted signaling proteins that have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis (1,2). WNT5A and WNT5B coordinate chondrocyte proliferation and differentiation by differentially regulating cyclin D1 and p130 expression, as well as chondrocyte-specific Col2a1 expression (3). Up-regulation of WNT5A and WNT5B has been observed in several types of human cancers, suggesting that they may be useful as diagnostic markers or potent therapeutic targets (4).

Molecular Weight: Predicted: 39 kDa

Observed: 465 kDa

Gene ID: 81029

NCBI Accession: NP_110402

UniProt: Q9H1J7

Pathways: WNT Signaling, Embryonic Body Morphogenesis, Positive Regulation of fat Cell Differentiation