RFX1 antibody (C-Term)

Catalog No. ABIN6992144

Product Details anti-RFX1 Antibody

Target: RFX1 (Regulatory Factor X, 1 (Influences HLA Class II Expression) (RFX1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist, this antibody will detect both isoforms.

Purification: RFX1 antibody is affinity chromatography purified via peptide column.

Immunogen: RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1. The immunogen is located within the last 50 amino acids of RFX1.

Isotype: IgG

Application Details

Application Notes: RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RFX1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RFX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for RFX1

Target: RFX1 (Regulatory Factor X, 1 (Influences HLA Class II Expression) (RFX1))

Alternative Name: RFX1 (RFX1 Products)

Synonyms: EFC antibody, RFX antibody, AI047719 antibody, AI385641 antibody, zgc:162914 antibody, regulatory factor X1 antibody, regulatory factor X, 1 (influences HLA class II expression) antibody, regulatory factor X, 1a (influences HLA class II expression) antibody, RFX1 antibody, Rfx1 antibody, rfx1a antibody

Background: RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain and the D region found in the C-terminal part of these proteins which facilitates dimerization (1,2). RFX1 is structurally related to regulatory factors X2, X3, X4, and X5 (3). RFX1 is a regulatory factor essential for MHC class II genes expression and can bind to an inverted repeat that is required for expression of hepatitis B virus genes (4). Recent study identifies RFX1 may play a tumor suppressor role in HCC as autophagy mediator (5).

Molecular Weight: Predicted: 108 kDa

Observed: 125 kDa

Gene ID: 5989

NCBI Accession: NP_002909

UniProt: P22670