Vinculin antibody (AA 1020-1134)

Catalog No. ABIN7428974

This Mouse Monoclonal antibody specifically detects Vinculin in WB. It exhibits reactivity toward Human.

Quick Overview for Vinculin antibody (AA 1020-1134) (ABIN7428974)

Target: Vinculin (VCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB)

Clone: C3

Product Details anti-Vinculin Antibody

Binding Specificity: AA 1020-1134

Purpose: Anti-Vinculin (VCL) Monoclonal Antibody

Specificity: The antibody is a mouse monoclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

Cross-Reactivity: Chicken, Cow, Dog, Guinea Pig, Mouse, Pig, Rabbit, Rat, Zebrafish (Danio rerio)

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Vinculin (VCL) corresdonding to Lys1020~Gln1134 with N-terminal His Tag

Application Details

Application Notes: Western blotting: 0.1-1 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: VCL

Background: MVCL, Metavinculin

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2