ATP4A antibody (AA 786-1014)

Catalog No. ABIN7436267

Product Details anti-ATP4A Antibody

Target: ATP4A (ATPase, H+/K+ Exchanging, alpha Polypeptide (ATP4A))

Binding Specificity: AA 786-1014

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)

Specificity: The antibody is a rabbit polyclonal antibody raised against ATP4a. It has been selected for its ability to recognize ATP4a in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) corresdonding to Pro786~Cys1014 (Accession # Q91WH7) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-ATP4A antibody (ABIN7436267)

Daher, Ducrot, Lefebvre, Zineeddine, Ausseil, Puy, Karim: "Crosstalk between Acidosis and Iron Metabolism: Data from In Vivo Studies." in: Metabolites, Vol. 12, Issue 2, (2022) (PubMed).

Target Details for ATP4A

Target: ATP4A (ATPase, H+/K+ Exchanging, alpha Polypeptide (ATP4A))

Alternative Name: ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4A Products)

Synonyms: ATP6A antibody, Hka antibody, Hkatpc antibody, ATPase H+/K+ transporting alpha subunit antibody, ATPase H+/K+ transporting alpha subunit L homeolog antibody, ATPase, H+/K+ exchanging, gastric, alpha polypeptide antibody, ATP4A antibody, Atp4a antibody, atp4a.L antibody

Background: Gastric H,K-ATPase Alpha Subunit, H(+)-K(+)-ATPase Alpha Subunit, Proton Pump, Potassium-transporting ATPase alpha chain 1

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process