SRD5A1 antibody

Catalog No. ABIN7430156

Product Details anti-SRD5A1 Antibody

Target: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRD5A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Steroid 5 Alpha Reductase 1 (SRD5a1)

Specificity: The antibody is a rabbit polyclonal antibody raised against SRD5a1. It has been selected for its ability to recognize SRD5a1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Steroid 5 Alpha Reductase 1 (SRD5a1) corresdonding to N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:150-600 Immunohistochemistry: 5-20 μg/mL

1:15-60 Immunocytochemistry: 5-20 μg/mL

1:15-60 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SRD5A1

Target: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Alternative Name: Steroid 5 Alpha Reductase 1 (SRD5A1 Products)

Synonyms: zgc:153768 antibody, 0610031P22Rik antibody, 4930435F02Rik antibody, S5AR 1 antibody, Srd5a-1 antibody, steroid 5 alpha-reductase 1 antibody, steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) antibody, 3-oxo-5-alpha-steroid 4-dehydrogenase 1 antibody, SRD5A1 antibody, srd5a1 antibody, Bm1_06570 antibody, CC1G_14953 antibody, Srd5a1 antibody

Background: 3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha 1

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process