SLC5A1 antibody (Internal Region)

Catalog No. ABIN7013885

Product Details anti-SLC5A1 Antibody

Target: SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SLC5A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: SGLT1

Sequence: KETIEIETQV PEKKK

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Application Details

Application Notes:

DS WB Results: Approx 75 kDa band observed in isolated Brush Border membranes from Human Kidney (calculated MW of 73.5 kDa according to NP_000334.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

DS IHC Results: In paraffin embedded Human Small Intestine shows staining of the glycocalix. Recommended concentration: 3-6 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunocytochemistry: Fig A.Frozen sections of Human Kidney show staining of the brush borders in DCT, while only weak background was observed in Rat and Pig Kidney. Recommended concentration: 2-4ug/ml. Fig B.PFA-fixed 4μm cryosections of Human Kidney shows staining of the brush border of proximal straight tubules (S3 segments) in the outer stripe zone. The signal drops to background levels upon blocking with the immunizing peptide.

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin. Aliquot and store at -20 °C. Minimize freezing and thawing.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Target Details for SLC5A1

Target: SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))

Alternative Name: SLC5A1 (SLC5A1 Products)

Synonyms: D22S675 antibody, NAGT antibody, SGLT1 antibody, zgc:66074 antibody, zgc:85782 antibody, SLC5A1 antibody, Sglt1 antibody, solute carrier family 5 member 1 antibody, solute carrier family 5 (sodium/glucose cotransporter), member 1 antibody, SLC5A1 antibody, slc5a1 antibody, Slc5a1 antibody

Background: SLC5A1, solute carrier family 5 (sodium/glucose cotransporter), member 1, D22S675, NAGT, SGLT1, Human Na+/glucose cotransporter 1 mRNA, complete cds, sodium/glucose cotransporter 1, solute carrier family 5 (sodium/glucose transporter), member 1

Gene ID: 6523